Metabolite and enzyme profiles of glycogen metabolism in Methanococcoides methylutens

Maitra, P. K. ; Bhosale, S. B. ; Kshirsagar, Deepa C. ; Yeole, T. Y. ; Shanbhag, Archana N. (2001) Metabolite and enzyme profiles of glycogen metabolism in Methanococcoides methylutens FEMS Microbiology Letters, 198 (1). pp. 23-29. ISSN 0378-1097

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...

Related URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10614.x

Abstract

When a buffered anaerobic cell suspension of Methanococcoides methylutens was maintained under methanol-limited conditions, intracellular glycogen and hexose phosphates were consumed rapidly and a very small amount of methane formed at 4 h of a starvation period. When methanol was supplemented after a total of 20 h of starvation, a reverse pattern was observed: the glycogen level and the hexose phosphate pool increased, and formation of methane took place after a lag period of 90 min. A considerable amount of methane was formed in 120 min after its detection with a rate of 0.18 µmol mg-1 protein min-1. When methane formation decreased after 270 min of incubation and finally came to a halt, probably due to complete assimilation of supplemented methanol, the levels of glycogen and hexose monophosphates decreased once again. However fructose 1,6-diphosphate levels showed a continuous increase even after exhaustion of methane formation. In contrast to the hexose phosphate pool, levels of other metabolites showed a small increase after addition of methanol. The enzyme profile of glycogen metabolism showed relatively high levels of triose phosphate isomerase. Glyceraldehyde 3-phosphate dehydrogenase reacted with NADPH with a three-fold higher activity as compared to that with NADH.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Microbiological Societies.
Keywords:Gluconeogenesis; Glycolysis; Glycolytic Metabolite; Methanogen; Methanococcoides Methylutens
ID Code:31411
Deposited On:25 Apr 2011 09:30
Last Modified:17 May 2016 14:03

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