A glucokinase from Saccharomyces cerevisiae

Maitra, P. K. (1970) A glucokinase from Saccharomyces cerevisiae Journal of Biological Chemistry, 245 (9). pp. 2423-2431. ISSN 0021-9258

PDF - Publisher Version

Official URL: http://www.jbc.org/content/245/9/2423.abstract


An ATP:D-glucose 6-phosphotransferase (EC or glucokinase from Saccharomyces cerevisiae is described. The enzyme has been purified about 100-fold from the crude extracts of a hexokinaseless mutant derived from a haploid strain of S. cerevisiae. The Km values for glucose and ATP are 28 and 50 µm, respectively. The maximal velocity of this enzyme toward fructose is 0.4% of that of glucose. The enzyme showed a marked heterogeneity when sedimented in a sucrose density gradient, its molecular weight ranging from 144,000 to a value in excess of 200,000. Yeast glucokinase shares a number of properties with yeast hexokinase (EC in the semiconstitutive nature of its synthesis with glucose, its broad pH optima, and inhibition with ADP and N-acetylglucosamine. Its mode of reaction with glucose and ATP, as indicated by initial velocity patterns, appears to be random.

Item Type:Article
Source:Copyright of this article belongs to American Society for Biochemistry and Molecular Biology.
ID Code:31302
Deposited On:25 Apr 2011 09:25
Last Modified:17 May 2016 13:54

Repository Staff Only: item control page