Evidence of a laminin binding protein on the surface of Leishmania donovani

Ghosh, Abhijit ; Kole, Labanyamoy ; Bandyopadhyay, Keya ; Sarkar, Kakali ; Das, Pijush K. (1996) Evidence of a laminin binding protein on the surface of Leishmania donovani Biochemical and Biophysical Research Communications, 226 (1). pp. 101-106. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1006/bbrc.1996.1317


Both the promastigote and amastigote forms of the intracellular parasite,Leishmania donovanibind the basement membrane glycoprotein laminin with high affinity (Kd= 3.56 × 10-9M and 3.98 × 10-9M respectively) with ~9000 and ~800 sites per cell. Bound laminin was identified by direct autoradiography and the binding protein through analysis of the parasite extract by SDS-PAGE and immunoblotting. A major component of 67 kDa was detected. The same protein was obtained when parasite outer membrane proteins were adsorbed to laminin-sepharose affinity matrix and subsequently eluted with SDS. The binding affinity of the isolated receptor was similar to that of the whole cells. Such a receptor isolated inLeishmaniafor the first time, may function as one of the bridging molecules for extracellular matrix recognition.

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