Inhibition of glutathione synthesis as a chemotherapeutic strategy for leishmaniasis

Kapoor, Preeti ; Sachdev, Monika ; Madhubala, Rentala (2000) Inhibition of glutathione synthesis as a chemotherapeutic strategy for leishmaniasis Tropical Medicine & International Health, 5 (6). pp. 438-442. ISSN 1360-2276

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Official URL: http://onlinelibrary.wiley.com/doi/10.1046/j.1365-...

Related URL: http://dx.doi.org/10.1046/j.1365-3156.2000.00565.x

Abstract

This study focuses on the use of buthionine sulphoximine (BSO), γ -glutamylcysteine synthetase inhibitor, on Leishmania donovani growth. The effect of BSO on amastigote multiplication within macrophages showed that 5 m m BSO decreased infectivity by about 50% and the mean number of amastigotes per 100 infected macrophages by 21%. The mechanism may be that BSO resulted in enhanced nitric oxide (NO) levels within macrophages, probably due to inhibition of GSH content since GSH (10 mm) given after BSO treatment led to a decrease in NO compared to macrophages treated with BSO alone which were preexposed to the Leishmania surface molecule lipophosphoglycan.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:Glutathione; L-buthionine Sulphoximine (BSO); Leishmania donovani; Nitric Oxide
ID Code:29861
Deposited On:23 Dec 2010 04:13
Last Modified:17 May 2016 12:39

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