Hepatic and extra-hepatic glutathione-S-transferase activity in wild pigeons (Columba livia)

Kumar, Ashwini ; Husain, M. M. ; Mukhtar, Hasan ; Krishna Murti, C. R. (1980) Hepatic and extra-hepatic glutathione-S-transferase activity in wild pigeons (Columba livia) Journal of Biosciences, 2 (3). pp. 181-189. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/2/181-189.pdf

Related URL: http://dx.doi.org/10.1007/BF02703244


Glutathione-S-transferase (EC activity was assayed in hepatic and extra-hepatic tissues of pigeons using l-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates. Gluthathione-S-transferase activity towards 1-chloro-2,4-dinitrobenzene in pigeon was in the order: kidney > liver > testes > brain > lung> heart. The enzyme activity with 1-chloro-2,4-dinitrobenzene as substrate was 40-44 times higher in pigeon liver and kidney than that observed with 1,2-dichloro-4-dinitrobenzene as substrate.K m values of hepatic and renal glutathione transferase with l-chloro-2,4-dinitrobenzene as substrate were 2.5 and 3 mM respectively. Double reciprocal plots with varying reduced gluthathione concentrations resulted in biphasic curves with two K m values (liver 0.31 mM and 4mM; kidney 0.36 mM and 1.3 mM). The enzyme activity was inhibited by oxidized gluthathione in a dose-dependent pattern. 3-Methylcholanthrene elicited about 50% induction of hepatic glutathione transferase activity whereas phenobarbital was ineffective.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Avian Glutathione-S-transferase; Oxidized Glutathione; 3-methylcholanthrene; Phenobarbital; Inhibition by Oxidized Glutathione; Glutathione-S-transferase
ID Code:29414
Deposited On:17 Dec 2010 08:07
Last Modified:17 May 2016 12:16

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