Preparation of bacterial enzymes by controlled lysis

Krishna Murti, C. R. (1960) Preparation of bacterial enzymes by controlled lysis Biochimica et Biophysica Acta, 45 . pp. 243-249. ISSN 0006-3002

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1. 1. Lysis of Vibrio cholerae and Escherichia coli under controlled conditions by Versene and lysozyme has been adapted to separate the cells into three major fractions. 2. 2. Succinic oxidase, cytochrome oxidase and DPNH-cytochrome c reductase are located in the protoplast membrane or sediment. 3. 3. TPN linked iso-citrate, G6P dehydrogenases, DPN-linked malic dehydrogenase, aldolase, aspartic acid deaminase, adenosine deaminase, histidase and iso-citritase activities are present in the lysates of the "protoplasts" obtained by this method.

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