Effect of L-methionine sulphoximine on the enzymes of nitrogen metabolism in barley leaves

Kumar, P. A. ; Abrol, Y. P. (1990) Effect of L-methionine sulphoximine on the enzymes of nitrogen metabolism in barley leaves Proceedings of the Indian Academy of Sciences, Section B , 100 (2). pp. 97-100. ISSN 0370-0097

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Official URL: http://www.ias.ac.in/j_archive/procb/volindex.html

Related URL: http://dx.doi.org/10.1007/BF03053431

Abstract

L-methionine sulphoximine, a potent inhibitor of glutamine synthetase decreased nitrate reductase activity by 50% at the end of 12h of treatment while nitrite reductase was insignificantly affected. By 3 h the inhibition of glutamine synthetase activity was complete. Elevated levels of ammonia induced by L-methionine sulphoximine did not influence glutamate dehydrogenase. It is inferred that ammonia accumulation does not affect photosynthetic electron transport which supplies reducing power to nitrite reductase. The failure of glutamate dehydrogenase activity to be induced by high ammonium levels shows that it is not involved in the process of ammonia assimilation in the leaves.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Ammonia; Methionine Sulphoximine; Nitrate Reductase; Nitrite Reductase; Glutamate Dehydrogenase; Barley
ID Code:28870
Deposited On:20 Dec 2010 08:09
Last Modified:10 May 2011 11:13

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