Isolation of isomaltose [6-(α-D-glucopyranosyl)D -glucose] from the culture filtrate of Penicillium chrysogenum Q-176

Giri, K. V. ; Saroja, K. ; Venkataraman, R. ; Rao, P. L. Narasimha (1954) Isolation of isomaltose [6-(α-D-glucopyranosyl)D -glucose] from the culture filtrate of Penicillium chrysogenum Q-176 Archives of Biochemistry and Biophysics, 51 (1). pp. 62-67. ISSN 0003-9861

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000398...

Related URL: http://dx.doi.org/10.1016/0003-9861(54)90453-1

Abstract

A method for the isolation of isomaltose [6-(α -D-glucopyranosyl) D-glucose] from the culture filtrates of Penicillium chrysogenum Q-176 using maltose as the carbohydrate substrate is described. The method is based on the complete conversion of maltose into isomaltose and other higher saccharides by the mold, followed by fractionation of the products formed in the culture filtrates on a column of charcoal (Norit A), water and 5% ethanol being used as solvents for elution of glucose and isomaltose. Characterization of the sugar by its derivatives is given. The carbohydrate composition of the fractions was determined by circular paper chromatographie technique.

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