A genetic assay for MRNA's of phage T4

Jayaraman, Ramamirtha ; Goldberg, Edward B. (1969) A genetic assay for MRNA's of phage T4 Proceedings of the National Academy of Sciences of the United States of America, 64 (1). pp. 198-204. ISSN 0027-8424

PDF - Publisher Version

Official URL: http://www.pnas.org/content/64/1/198.abstract?sid=...


A method has been developed by which many gene-specific mRNA's in T4-infected cells can be quantitatively assayed. The method involves separation of complementary strands of phage T4 DNA, hybridization of the strands with RNA, digestion of nonhybridized regions of DNA with an endonuclease specific for single-stranded DNA, and assay of protected genetic markers by transformation. It has been shown that the gene γIIB is transcribed early from the light strand and that the gene 21 is transcribed late from the heavy strand.

Item Type:Article
Source:Copyright of this article belongs to National Academy of Sciences, USA.
ID Code:28478
Deposited On:15 Dec 2010 12:00
Last Modified:17 May 2016 11:35

Repository Staff Only: item control page