Wider, G. ; Macura, S. ; Anil Kumar, ; Ernst, R. R. ; Wuthrich, K. (1984) Homonuclear two-dimensional 1H NMR of proteins. Experimental procedures Journal of Magnetic Resonance (1969), 56 (2). pp. 207-234. ISSN 0022-2364
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002223...
Related URL: http://dx.doi.org/10.1016/0022-2364(84)90099-4
Abstract
Experimental techniques used for homonuclear 2D 1H NMR studies of proteins are described. A brief survey of the general strategy for structural studies of proteins by 2D NMR is included. The main part of the paper discusses guidelines for the selection of experimental techniques, the elimination of artifacts and unwanted peaks in protein 2D 1H NMR spectra, suppression of the solvent line in H2O solutions, experimental parameters, numerical data processing before and after Fourier transformation, and suitable presentations of complex 2D NMR spectra.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 27936 |
| Deposited On: | 13 Dec 2010 08:31 |
| Last Modified: | 12 May 2011 07:27 |
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