An in vitro system to study drug sensitivity of Mycobacterium leprae using infected human tissue

Vithala, Lakshmi ; Talati, Swati ; Mahadevan, P. R. (1983) An in vitro system to study drug sensitivity of Mycobacterium leprae using infected human tissue Journal of Biosciences, 5 (3). pp. 235-241. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/5/235-241.pdf

Related URL: http://dx.doi.org/10.1007/BF02716606

Abstract

A reliable screening technique for assessing the sensitivity of Mycobacterium leprae to drugs has been developed. The method is based on the susceptibility or otherwise of M. leprae- infected tissues from lepromatous leprosy patients to the action of diaminodiphenyl sulphone (dapsone) or rifampicin on the incorporation of [14C]-acetate into lipids. The extent of inhibition or lack of inhibition correlated very well with the drug sensitivity or resistance of the bacteria isolated from the patients to the above drugs. A similar trend was observed when the incorporation into individual fractions of neutral lipids was measured. There was no incorporation by heat-killed tissues. This method correlates well with the 3,4-dihydroxyphenylalanine uptake studies.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Drug Sensitivity; Resistance; Mycobacterium leprae; [14c]-Acetate Uptake
ID Code:27663
Deposited On:10 Dec 2010 11:53
Last Modified:17 May 2016 10:52

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