Delineation and quantitation of human peripheral blood lymphocyte subpopulations by electrophoretic mobility and role of surface charge in cell to cell interaction

Hanjan, S. N. S. ; Talwar, Gursaran Parshad ; Kidwai, Zeba ; Indira Nath, (1977) Delineation and quantitation of human peripheral blood lymphocyte subpopulations by electrophoretic mobility and role of surface charge in cell to cell interaction The Journal of Immunology, 118 (1). 235 -241. ISSN 0022-1767

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Official URL: http://www.jimmunol.org/cgi/content/abstract/118/1...

Abstract

A modified procedure is proposed for determination of electrophoretic mobility (EPM) of lymphocytes which enables readings on 200 cells from a sample size of 5 x 106 lymphocytes. In normal human peripheral blood, B lymphocytes (bearing surface immunoglobulin and receptor for activated C3) were found to carry the lowest charge (0.94 ± 0.05 μ/sec/V/cm). Their number on the EPM basis was 16.9 ± 4.2%. Two subpopulations of T lymphocytes, one carrying high charge (TH) with a mean EPM of 1.26 ± 0.04 μ /sec/V/cm and the other carrying lower charge (TL) with a mean EPM of 1.11 ± 0.03 μ /sec/V/cm were discernible. The former subpopulation of T cells formed spontaneous rosettes with SRBC in 10 min and the latter represented the remaining T lymphocytes which formed rosettes with 24 hr of incubation with SRBC. The number of early rosette-forming cells increased from 69 to 89% when T cells were incubated with neuraminidase-treated SRBC. These observations suggest that complementary electrostatic charge on T cells and SRBC are among the forces facilitating formation of rosettes between these cells.

Item Type:Article
Source:Copyright of this article belongs to American Association of Immunologists.
ID Code:27637
Deposited On:10 Dec 2010 11:55
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