Growth of gold nanoparticles in human cells

Anshup, ; Sai Venkataraman, J. ; Subramaniam, Chandramouli ; Rajeev Kumar, R. ; Priya, Suma ; Santhosh Kumar, T. R. ; Omkumar, R. V. ; John, Annie ; Pradeep, T. (2005) Growth of gold nanoparticles in human cells Langmuir, 21 (25). pp. 11562-11567. ISSN 0743-7463

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Gold nanoparticles of 20-100 nm diameter were synthesized within HEK-293 (human embryonic kidney), HeLa (human cervical cancer), SiHa (human cervical cancer), and SKNSH (human neuroblastoma) cells. Incubation of 1 mM tetrachloroaurate solution, prepared in phosphate buffered saline (PBS), pH 7.4, with human cells grown to ~80% confluency yielded systematic growth of nanoparticles over a period of 96 h. The cells, stained due to nanoparticle growth, were adherent to the bottom of the wells of the tissue culture plates, with their morphology preserved, indicating that the cell membrane was intact. Transmission electron microscopy of ultrathin sections showed the presence of nanoparticles within the cytoplasm and in the nucleus, the latter being much smaller in dimension. Scanning near field microscopic images confirmed the growth of large particles within the cytoplasm. Normal cells gave UV-visible signatures of higher intensity than the cancer cells. Differences in the cellular metabolism of cancer and noncancer cells were manifested, presumably in their ability to carry out the reduction process.

Item Type:Article
Source:Copyright of this article belongs to American Chemical Society.
ID Code:27463
Deposited On:10 Dec 2010 12:18
Last Modified:08 Jun 2011 11:32

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