A bias in the α β t cell receptor variable region gene usage in takayasu's arteritis

Nityanand, S. ; Giscombe, R. ; Srivastava, S. ; Hjelmstorm, P. ; Sanjeevi, C. B. ; Sinha, N. ; Grunewald, J. ; Lefvert, A. K. (1997) A bias in the α β t cell receptor variable region gene usage in takayasu's arteritis Clinical & Experimental Immunology, 107 (2). pp. 261-268. ISSN 0009-9104

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-...

Related URL: http://dx.doi.org/10.1111/j.1365-2249.1997.295-ce1186.x


Takayasu's arteritis (TA) is a chronic large vessel vasculitis with a predilection for the aortic arch and its branches. T lymphocytes may be important in the pathogenesis, as they have been found to infiltrate the vascular lesions. To elucidate further the role of T cells in the disease, we studied circulating CD4+ and CD8+ T cells, expression of the activation marker (HLA-DR), marker for naive (CD45RA) and primed (CD45RO) cells and the different variable α/ β (AV/BV) gene segments on them. The TCR AV/BV repertoire was studied using a panel of 15 T cell receptor (TCR) V-specific MoAbs by flow cytometry in 18 patients and 23 age- and sex-matched controls. Patients had a higher percentage of AV12S1 (P < 0.05), BV6S7 (P < 0.05) and BV9 (P < 0.001)-bearing CD4+ cells. Patients also had a higher frequency of expansions, i.e. of T cell populations with an abnormally high TCR AV/BV gene usage. In patients' CD4+ subset of cells, there were 22 expansions out of 231 analyses (9.5%), whereas in controls, four were expanded out of 310 analyses (1%) (P < 0.001). For CD8+ cells, the frequency of expansions was 32 in 231 analyses (14%) in patients and nine out of 304 analyses in controls (3%) (P < 0.01). In addition, there was a correlation between CD4+ expansions and disease activity; nine out of 10 patients with active disease in comparison with two out of eight patients with inactive disease (P < 0.01) had an expansion. Some of the expanded populations in patients were phenotypically characterized and observed to be HLA-DR<, CD28<, CD45RA< and CD45RO+, with a greater proportion being CD45RO+. Patients had a higher percentage of expression of HLA-DR on both CD4+ and CD8+ T cells (P < 0.01). The percentages of naive and primed CD4+ and CD8+ T cells, γ δ+ T cells and natural killer cells were comparable to those in the control group.

Item Type:Article
Source:Copyright of this article belongs to John Wiley & Sons.
Keywords:Lymphocytes; T Cell Receptor; Takayasu's Arteritis
ID Code:27160
Deposited On:08 Dec 2010 12:36
Last Modified:17 May 2016 10:25

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