Inhibition of DNA gyrase activity by Mycobacterium smegmatis MurI

Sengupta, Sugopa ; Nagaraja, Valakunja (2008) Inhibition of DNA gyrase activity by Mycobacterium smegmatis MurI FEMS Microbiology Letters, 279 (1). pp. 40-47. ISSN 0378-1097

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...

Related URL: http://dx.doi.org/10.1111/j.1574-6968.2007.01005.x

Abstract

Glutamate racemase (MurI) catalyzes the interconversion of L-glutamate to D-glutamate, one of the essential amino acids present in the peptidoglycan. In addition to this essential enzymatic function, MurI from Escherichia coli, Bacillus subtilis and Mycobacterium tuberculosis inhibit DNA gyrase activity. A single gene for murI found in the Mycobacterium smegmatis genome was cloned and overexpressed in a homologous expression system to obtain a highly soluble enzyme. In addition to the racemization activity, M. smegmatis MurI inhibits DNA gyrase activity by preventing DNA binding of gyrase. The sequestration of the gyrase by MurI results in inhibition of all reactions catalyzed by DNA gyrase. More importantly, MurI overexpression in vivo in mycobacterial cells provides protection against the action of ciprofloxacin. The DNA gyrase-inhibitory property thus appears to be a typical characteristic of MurI and would have probably evolved to either modulate the function of the essential housekeeping enzyme or to provide protection to gyrase against gyrase inhibitors, which cause double-strand breaks in the genome.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Microbiological Societies.
Keywords:Mycobacterium Smegmatis; Glutamate Racemase; DNA Gyrase; Gyrase Inhibitor; Protein-protein Interaction
ID Code:27026
Deposited On:08 Dec 2010 12:50
Last Modified:17 May 2016 10:19

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