Saravanan, Matheshwaran ; Elango, Kathirvel ; Chandrashekaran, Siddamadappa ; Anand, Narayanaswamy ; Nagaraja, Valakunja (2003) A new type II restriction endonuclease, OfoI from nonheterocystous cyanobacterium Oscillatoria foreaui Current Science, 85 (2). pp. 188-191. ISSN 0011-3891
![[img]](http://repository.ias.ac.in/style/images/fileicons/application_pdf.png)
|
PDF
- Publisher Version
2MB |
Official URL: http://www.ias.ac.in/currsci/jul252003/188.pdf
Abstract
Although restriction enzymes are widely distributed in nature, many bacterial genera are yet to be explored for the presence of this important class of enzymes. We have purified and characterized a new type II restriction endonuclease, OfoI from a nonheterocyst cyanobacterium Oscillatoria foreaui. The recognition sequence has been determined by primer extension analysis. The purified enzyme OfoI recognizes and cleaves the palindromic hexanucleotide 5'-C↓YCGRG-3', generating 5'-protruding ends.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Current Science Association. |
| ID Code: | 27022 |
| Deposited On: | 08 Dec 2010 12:50 |
| Last Modified: | 17 May 2016 10:19 |
Repository Staff Only: item control page
