Diamine oxidase of Lathyrus sativus seedlings

Suresh, M. Rangarajan ; Ramakrishna, Seethala ; Adiga, P. Radhakantha (1976) Diamine oxidase of Lathyrus sativus seedlings Phytochemistry, 15 (4). pp. 483-485. ISSN 0031-9422

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00319...

Related URL: http://dx.doi.org/10.1016/S0031-9422(00)88952-9


A diamine oxidase has been purified (ca 40-fold) from 5-day old etiolated seedlings of L. sativus by MnCl2 treatment, (NH4)2SO4 and Me2CO fractionations, positive adsorption on alumina Cγ -gel followed by column chromatography on DEAE-Sephadex. This cytosol enzyme oxidatively deaminates a number of aliphatic and aryl alkylamines but not histamine. NSD-1055, semicarbazide and other carbonyl reagents, α ,α 'bipyridyl, 1,10-phenanthroline and 8-hydroxyquinoline inhibit the enzyme. Pargyline, SKF trans-385, atabrine were without effect on the enzyme.

Item Type:Article
Source:Copyright of this article belongs to Phytochemical Society of Europe.
Keywords:Lathyrus sativus; Leguminosae; Chick-pea; Diamine Oxidase; Distribution; Purification; Putrescine; Cadaverine; Spermidine; Spermine
ID Code:26828
Deposited On:08 Dec 2010 13:07
Last Modified:13 May 2011 05:24

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