Sri Venugopal, K. S. ; Adiga, P. R. (1980) A simple procedure for purification of N-carbamoylputrescine: application to assays of putrescine transcarbamoylase and agmatine iminohydrolase activities Analytical Biochemistry, 104 (2). pp. 440-444. ISSN 0003-2697
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...
Related URL: http://dx.doi.org/10.1016/0003-2697(80)90096-2
Abstract
The observation that N-carbamoylputrescine is quantitatively excluded on O-(carboxymethyl)-cellulose columns with simultaneous retention of putrescine and agmatine has been utilized to develop a sensitive radiometric assay for putrescine transcarbamoylase and a colorimetric assay for agmatine iminohydrolase. A simple procedure for obtaining bulk amounts of pure synthetic N-carbamoylputrescine by separation from putrescine and dicarbamoylputrescine on Dowex 50 (Na+) resin is described.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 26788 |
| Deposited On: | 08 Dec 2010 13:11 |
| Last Modified: | 13 May 2011 05:08 |
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