Antigenic similarities among biotin-binding proteins

Subramanian, N. ; Subramanian, Sarada ; Adiga, P. Radhakantha (1996) Antigenic similarities among biotin-binding proteins IUBMB Life, 40 (4). pp. 663-670. ISSN 1521-6543

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Using polyclonal antisera to avidin, streptavidin, and biotin binding protein-II (BBP-II), the extents of immunological cross-reactivities shared by this family of biotin-binders were investigated. In Western blot analysis, varying degrees of cross-reactivities among these proteins depending on the antisera used was observed. Using liquid phase radioimmunoassay, evidence for the existence of common antigenic determinants shared by all the biotin-binders was deduced. Antibodies directed against such epitopes inhibited the binding of [3H]biotin to as well as displace the bound ligand from all the three high affinity biotin-binders in a dose dependent manner.

Item Type:Article
Source:Copyright of this article belongs to International Union of Biochemistry and Molecular Biology.
Keywords:Biotin-binders; Antibodies; Ligand-binding; Inhibition
ID Code:26781
Deposited On:08 Dec 2010 13:12
Last Modified:13 May 2011 04:12

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