Subramanian, N. ; Subramanian, Sarada ; Adiga, P. Radhakantha (1996) Antigenic similarities among biotin-binding proteins IUBMB Life, 40 (4). pp. 663-670. ISSN 1521-6543
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Official URL: http://www3.interscience.wiley.com/journal/1178875...
Related URL: http://dx.doi.org/10.1080/15216549600201263
Abstract
Using polyclonal antisera to avidin, streptavidin, and biotin binding protein-II (BBP-II), the extents of immunological cross-reactivities shared by this family of biotin-binders were investigated. In Western blot analysis, varying degrees of cross-reactivities among these proteins depending on the antisera used was observed. Using liquid phase radioimmunoassay, evidence for the existence of common antigenic determinants shared by all the biotin-binders was deduced. Antibodies directed against such epitopes inhibited the binding of [3H]biotin to as well as displace the bound ligand from all the three high affinity biotin-binders in a dose dependent manner.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to International Union of Biochemistry and Molecular Biology. |
| Keywords: | Biotin-binders; Antibodies; Ligand-binding; Inhibition |
| ID Code: | 26781 |
| Deposited On: | 08 Dec 2010 13:12 |
| Last Modified: | 13 May 2011 04:12 |
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