Comparison of biotin binding protein of pregnant rat serum with rat serum albumin

Seshagiri, P. B. ; Adiga, P. R. (1989) Comparison of biotin binding protein of pregnant rat serum with rat serum albumin Journal of Biosciences, 14 (3). pp. 221-231. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/14/221-231.pdf

Related URL: http://dx.doi.org/10.1007/BF02716682

Abstract

The purified biotin binding protein of pregnant rat serum was shown to be immunologically similar to rat serum albumin as assessed by a sensitive radioimmunoassay. In radioimmunoassay for rat biotin binding protein, the binding of [125I] rat biotin binding protein to anti-chicken egg yolk biotin binding protein antibodies was displaced by both rat serum (10-100 nl) and purified rat serum albumin (0.1-10 ng). Similarly, in radioimmunoassay for rat serum albumin the binding of [125I] rat serum albumin to either anti-rat serum albumin antibodies or anti-chicken egg yolk biotin binding protein antibodies was displaced by unlabelled rat biotin binding protein at comparable concentration range (0.5-10 ng). Significant fractions of radioiodinated rat biotin binding protein and rat serum albumin bound to antibodies to chicken egg yolk biotin binding protein. In immature rats, the circulating half-lives of rat biotin binding protein and rat serum albumin were determined to be 12 and 17 h respectively. The rat biotin binding protein and rat serum albumin were analysed by techniques that exploit their physicochemical properties. They displayed similar electrophoretic mobilities in alkaline as well as denaturing sodium dodecyl sulphate-polyacrylamide gels. However, in nonequilibrium pH gradient polyacrylamide gel electrophoresis, they resolved clearly. In two-dimensional tryptic peptide map analysis, the two proteins showed similarities as well as significant differences in the relative distribution patterns of their iodopeptides. These results showed that the primary structure of rat biotin binding protein and rat serum albumin were different in finer details despite the fact that they shared significant immunological cross-reactivity.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Biotin Binding Protein ( Chicken; Rat); Rat Serum Albumin; Immunological-crossreactivity
ID Code:26756
Deposited On:08 Dec 2010 13:14
Last Modified:17 May 2016 10:03

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