Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings

Abstract

An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD⁺ with concomitant liberation of NH₃ was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with K_m 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.