Structural analysis of the glycosylinositol phospholipid anchors of membrane proteins

Mayor, Satyajit ; Menon, Anant K. (1990) Structural analysis of the glycosylinositol phospholipid anchors of membrane proteins Methods, 1 (3). pp. 297-305. ISSN 1046-2023

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S10462...

Related URL: http://dx.doi.org/10.1016/S1046-2023(05)80331-7

Abstract

We describe methods for the characterization of glycosylinositol phospholipid (GIPL) anchors of membrane proteins isolated from metabolically labeled cells. The analyses involve chemical and enzymatic fragmentation of metabolically labeled GIPLs, followed by the separate analysis of the glycan and lipid components.

Item Type:Article
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ID Code:26706
Deposited On:08 Dec 2010 13:21
Last Modified:08 Dec 2010 13:21

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