Cloning and expression in Escherichia coli of a recA-like gene from Vibrio cholerae

Paul, Kalidas ; Ghosh, Subrata K. ; Das, Jyotirmoy (1986) Cloning and expression in Escherichia coli of a recA-like gene from Vibrio cholerae Molecular and General Genetics, 203 (1). pp. 58-63. ISSN 0026-8925

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Official URL: http://www.springerlink.com/content/v948g31432j846...

Related URL: http://dx.doi.org/10.1007/BF00330384

Abstract

A library containing more than 80% of the Vibrio cholerae genome was constructed by cloning BamH1 restriction fragments into pBR322. Using interspecific complementation of an Escherichia coli recA mutant with plasmids containing the gene bank of V. cholerae, a recA-like gene was identified. The recombinant plasmid, designated as pDP145, contained a 1.45 kb segment of V. cholerae DNA which codes for a protein of molecular weight 39,000. The product of this gene confers methyl methane sulphonate resistance on the E. coli recA mutant, suppresses its ultraviolet (UV) light sensitive phenotype and has proteolytic activity on the phage λ repressor. Induction of a 39,000 dalton protein in UV-irradiated V. cholerae cells was demonstrated.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Genomic Library; Recombinant Plasmid pDP145; recA Protein; SOS Induction; UV Sensitivity
ID Code:26558
Deposited On:08 Dec 2010 13:34
Last Modified:09 Feb 2011 08:28

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