Das, Goutam ; Das, Jyotirmoy (1983) Radiation-sensitive mutant of hypertoxinogenic strain 569B of Vibrio cholerae Mutation Research/ Fundamental and Molecular Mechanisms of Mutagenesis, 109 (1). pp. 21-30. ISSN 0027-5107
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002751...
Related URL: http://dx.doi.org/10.1016/0027-5107(83)90091-X
Abstract
A radiation-sensitive mutant of the hypertoxinogenic strain 569B of Vibriocholerae was isolated and characterized. The mutant, designated V. cholerae 569Bs, lacks both excision- and medium-dependent dark-repair mechanisms of UV-induced DNA damage while retaining the wild-type photoreactivating capability. Analysis of the UV-irradiated cell DNA by velocity sedimentation in alkaline sucrose gradient suggests that UV-induced pyrimidine dimers may not be incised in these cells. In contrast to the wild-type cells, the mutant cell DNA was degraded after treatment with nalidixic acid. The mutant cells failed to produce any detectable amount of cholera toxin as measured by ileal-loop assay.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 26545 |
| Deposited On: | 08 Dec 2010 13:35 |
| Last Modified: | 09 Feb 2011 08:32 |
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