Dark and light repair in ultraviolet-irradiated Acholeplasma laidlawii

Das, Jyotirmoy ; Maniloff, Jack ; Bhattacharjee, Sukhendu B. (1972) Dark and light repair in ultraviolet-irradiated Acholeplasma laidlawii Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 259 (2). pp. 189-197. ISSN 0005-2787

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...

Related URL: http://dx.doi.org/10.1016/0005-2787(72)90058-5


The ultraviolet sensitivity and repair capability of Acholeplasma laidlawii were investigated. Ultraviolet sensitivity and degree of photoreversal vary with the cells' growth phase and pass through a maximum for middle to late logarithmic phase cells. The cells also show a liquid holding recovery after irradiation. Chloramphenicol, added either before or after the ultraviolet dose, reduces the recovery by a factor of 10. Nalidixic acid has a similar effect if added before the ultraviolet, but, if added after the ultraviolet dose, the reduction in recovery is much less. Hence, liquid holding recovery may involve a DNA repair enzyme. Alkaline sucrose analysis of the cells' DNA during recovery shows the appearance of breaks and their repair. The DNA profiles also show a small molecular weight component, which may be the virus which has been isolated from these cells (A. LISS and J.Maniloff , Science, 173 (1971) 725).

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