Balganesh, Meenakshi ; Das, Jyotirmoy (1979) Transfection of Vibrio cholerae by bacteriophage Φ149 DNA Biochemical and Biophysical Research Communications, 90 (3). pp. 726-733. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...
Related URL: http://dx.doi.org/10.1016/0006-291X(79)91888-6
Abstract
DNA isolated from Choleraphage Φ149 of Group IV was infectious when mixed with competent V. cholerae cells. The cells were competent during mid-log phase of growth. The infectivity of phage DNA was destroyed by deoxyribonuclease but not by ribonuclease or pronase. About 5 min is required for the establishment of the DNase resistant state. The dose response curve for transfection suggested that 2 to 3 molecules of DNA are required to produce one infections center. An infectivity of 5 × 104 infectious center per µg of DNA was obtained.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 26529 |
| Deposited On: | 08 Dec 2010 13:36 |
| Last Modified: | 09 Feb 2011 08:39 |
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