Transfection of Vibrio cholerae by bacteriophage Φ149 DNA

Balganesh, Meenakshi ; Das, Jyotirmoy (1979) Transfection of Vibrio cholerae by bacteriophage Φ149 DNA Biochemical and Biophysical Research Communications, 90 (3). pp. 726-733. ISSN 0006-291X

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DNA isolated from Choleraphage Φ149 of Group IV was infectious when mixed with competent V. cholerae cells. The cells were competent during mid-log phase of growth. The infectivity of phage DNA was destroyed by deoxyribonuclease but not by ribonuclease or pronase. About 5 min is required for the establishment of the DNase resistant state. The dose response curve for transfection suggested that 2 to 3 molecules of DNA are required to produce one infections center. An infectivity of 5 × 104 infectious center per µg of DNA was obtained.

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ID Code:26529
Deposited On:08 Dec 2010 13:36
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