Mechanism of action of amphotericin B on Leishmania donovani promastigotes

Saha, Ahindra Kumar ; Mukherjee, Tanmoy ; Bhaduri, Amar (1986) Mechanism of action of amphotericin B on Leishmania donovani promastigotes Molecular and Biochemical Parasitology, 19 (3). pp. 195-200. ISSN 0166-6851

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The growth of Leishmania donovani promastigotes in a liquid medium was completely inhibited by amphotericin B at a concentration of 0.3 μm ml-1 (0.3 μM). Continuous release of small molecules that absorb at 260 nm and 280 nm was observed after contact with the drug. Uptake of [U-14C] glucose was inhibited in cells treated with the drug. An immediate release of isotopic glucose and its metabolites from preloaded cells could be demonstrated after incubation with amphotericin B (0.4 μM). Inhibition of respiration by the drug was a comparatively slower process. All the above effects could be effectively prevented in the presence of either cholesterol or ergosterol. The primary site of action of amphotericin B on L. donovani promastigote cells appears to be membrane sterols that result in a loss of the permeability barrier to small metabolites. An interesting biochemical similarity, thus, emerges between flagellated protozoa and fungi.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Leishmania Donovani; Amphotericin B; Polyene Antibiotics; Protozoa
ID Code:26429
Deposited On:06 Dec 2010 12:33
Last Modified:16 May 2011 10:18

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