Studies on microbial protein synthesis with Azotobacter vinelandii as the test organism I. Incorporation of radioactive phosphate and sulfate into the resting cells in the presence of antibiotics and antimetabolites

Chakravorty, Maharani ; Burma, D. P. (1962) Studies on microbial protein synthesis with Azotobacter vinelandii as the test organism I. Incorporation of radioactive phosphate and sulfate into the resting cells in the presence of antibiotics and antimetabolites Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 55 (1-2). pp. 110-119. ISSN 0926-6550

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/092665...

Related URL: http://dx.doi.org/10.1016/0926-6550(62)90222-0

Abstract

The effects of the three antibiotics, chloramphenicol, streptomycin and penicillin and two antimetabolites, p-fluorophenylalanine and 8-azaguanine, on the synthesis of nucleic acid and protein in Azotobacter vinelandii were investigated by studying the kinetics of incorporation of radioactive phosphate and sulfate into the resting cells. The results are in general agreement with those obtained with other microorganisms and indicate that nucleic acid plays a direct part in protein synthesis and that the reverse phenomenon does not occur.

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