Incorporation of ₅S RNA into ₁₆S·₂₃S RNA complex

Abstract

₅S RNA as such is not incorporated into ₁₆S·₂₃S RNA complex formed under reconstitution condition. However, the addition of 50S ribosomal proteins, L₅, L₁₈ and L₂₅/L₁₅ results in its incorporation in stoichiometric amount. None of the proteins added individually is capable of incorporating ₅S RNA into the complex. Of the different combinations in pairs that are possible out of the four proteins, the pairs L₅, L₁₈ and L₁₅, L₁₈ stimulate the incorporation to some extent. Of the four possible triplets, L₅, L₁₈, L₂₅ or L₅, L₁₅, L₁₈ is the most efficient for maximum incorporation of ₅S RNA. The presence of all the four proteins is no more effective than the combinations of the three.