The biosynthesis of uridine diphosphate glucose and sucrose in sugar beet leaf

Burma, D. P. ; Mortimer, D. C. (1956) The biosynthesis of uridine diphosphate glucose and sucrose in sugar beet leaf Archives of Biochemistry and Biophysics, 62 (1). pp. 16-28. ISSN 0003-9861

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1. 1. The mechanisms for biosynthesis of UDPG and sucrose in sugarbeet leaf and leaf homogenate have been investigated by providing radioactive G-1-P with various cofactors and identifying the radioactive products after short time intervals. When radioactive G-1-P is fed to an excised leaf, sucrose equally labeled in both moieties is formed. When UTP is fed with G-1-P, the yield of radioactive sucrose is reduced, and a relatively large proportion of the radioactive glucose is converted to UDPG. 2. 2. The leaf homogenate converts radioactive G-1-P to radioactive glucose and fructose. A mixture of radioactive G-1-P and UTP is converted to radioactive UDPG, and UDP plus ATP can replace UTP. A method for preparation and purification of radioactive UDPG is outlined. 3. 3. Radioactive sucrose, labeled in the glucose moiety, is biosynthesized by leaf homogenates during 15 min. incubation when radioactive G-1-P plus UTP and F-6-P are added in appropriate concentrations. There is no reaction when UTP is omitted, and fructose or F-1,6-P cannot replace F-6-P. However, UDPG does replace G-1-P plus UTP for sucrose synthesis. Traces of radioactive sucrose phosphate are found in those systems which form sucrose. 4. 4. These data show that sucrose is biosynthesized in the sugar-beet leaf as the product of a sequence of reactions, in which UDPG, formed from G-1-P and UTP, reacts with F-6-P to give sucrose phosphate. The sucrose phosphate is rapidly dephosphorylated, and UTP is regenerated from UDP by ATP.

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