Interaction of colchicine analogues with purified tubulin

Choudhury, Goutam Ghosh ; Banerjee, Ashok ; Bhattacharyya, Bhabatarak ; Biswas, Birendra B. (1983) Interaction of colchicine analogues with purified tubulin FEBS Letters, 161 (1). pp. 55-59. ISSN 0014-5793

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Binding of two colchicine analogues, desacetamidocolchicine and 2-methoxy-5-(2',3',4'-trimethoxyphenyl) tropone to purified tubulin have been studied. Both analogues bind to tubulin with a significant increase in fluorescence polarization of the drugs in solutions containing tubulin. The Kd for tubulin-drug complexes were found to be 1.25 × 10-6 M and 1.08 × 10-6 M for desacetamidocolchicine and 2-methoxy-5-(2',3',4'-trimethoxyphenyl) tropone, respectively. Scatchard analysis of the fluorescence titration curve of drug tubulin interaction also gives the values of stoichiometry and affinity constant. These were 0.8 and 1.6 × 106 M-1 for desacetamidocolchicine, and 0.9 and 0.58 × 106 M-1 for 2-methoxy-5-(2',3',4'-trimethoxyphenyl) tropone.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:Brain Tubulin; B-ring of Colchicine; Fluorescence; Polarization; Binding Parameter
ID Code:26173
Deposited On:06 Dec 2010 12:58
Last Modified:17 May 2016 09:30

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