Tubulin subunit carboxyl termini determine polymerization efficiency

Sackett, D. L. ; Bhattacharyya, B. ; Wolff, J. (1985) Tubulin subunit carboxyl termini determine polymerization efficiency Journal of Biological Chemistry, 260 (1). pp. 43-45. ISSN 0021-9258

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Official URL: http://www.jbc.org/content/260/1/43.abstract?sid=b...


Cleavage of tubulin by subtilisin removes a small (Mr < 2000) fragment from the C-terminal end of both α and β subunits. The resulting protein is much reduced in negative charge. The cleaved, less acidic protein retains its competence to polymerize in a GTP-dependent and cold-, GDP-, and podophyllotoxin-sensitive manner and assembles into sheets or bundles of twisted filaments. The critical concentration for polymerization of the cleaved protein is about 50-fold lower than that for intact tubulin. It is proposed that the C termini of the subunits normally impede polymerization.

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Source:Copyright of this article belongs to American Society for Biochemistry and Molecular Biology.
ID Code:26147
Deposited On:06 Dec 2010 13:01
Last Modified:17 May 2016 09:29

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