Role of B-ring of colchicine in its binding to Zn(II)-induced tubulin-sheets

Banerjee, Asok ; Maity, Sankar N. ; Chaudhuri, Sukla Ray ; Bhattacharyya, B. (1987) Role of B-ring of colchicine in its binding to Zn(II)-induced tubulin-sheets Journal of Biosciences, 11 (1-4). pp. 525-536. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/11/525-536.pdf

Related URL: http://dx.doi.org/10.1007/BF02704701

Abstract

Colchicine-tubulin dimer complex, a potent inhibitor of normal microtubule assembly undergoes extensive self-assembly in the presence of 1 X 10-4 M zinc sulphate. Polymers assembled from colchicine-tubulin dimer complexes are sensitive to cold. Although colchicine can be accomodated within the polymeric structure, the drug cannot bind to tubulin subunits in the intact polymers. This is evidenced by the fact that (a) the colchicine binding activity of tubulin is lost when allowed to polymerize with zinc sulphate, (b) the loss in colchicine binding could be prevented by preincubation of tubulin with 1 X 10-3 M CaCl2 or 1 X 10-5 M vinblastine sulphate and finally (c) no loss in colchicine binding activity is found when tubulin is kept at a concentration far below the critical concentration for polymerization. Unlike colchicine, its B-ring analogues desacetamido colchicine (devoid of the B-ring subtituent) and 2-methoxy-5-(2', 3', 4'-trimethoxyphenyl) tropone (devoid of the B-ring) can bind to tubulin subunits in the intact polymers. Thus we conclude that the colchicine binding domain on the tubulin molecule is mostly (if not completely) exposed in the Zn(II) -induced polymers and the B-ring substituent plays a major role in determining the binding ability of a colchicine analogue to tubulin in the intact Zn(II) -induced sheets.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Colchicine-tubulin Dimer Complex; B-ring; Tubulin Sheets
ID Code:26137
Deposited On:06 Dec 2010 13:02
Last Modified:17 May 2016 09:28

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