In vivo survival and stratification of cultured limbal epithelium

Fatima, Anees ; Vemuganti, Geeta K. ; Iftekhar, Ghazala ; Rao, Gullapalli N. ; Sangwan, Virender S. (2007) In vivo survival and stratification of cultured limbal epithelium Clinical and Experimental Optometry, 35 (1). pp. 96-98. ISSN 0816-4622

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A 6-year-old Bangladeshi girl presented with total limbal stem cell deficiency in the left eye, secondary to a 6-month-old chemical injury. The patient had also previously undergone two limbal transplantation surgeries. At the authors' centre the child underwent autologous cultured limbal epithelium transplantation, on human amniotic membrane, without the use of air-lift technique. Symptomatic relief, re-epithelialization of the ocular surface, regression of corneal pannus and slight improvement in vision were all noted. The corneal button obtained at the time of keratoplasty (performed 4 months later) revealed stratified epithelium with basement membrane. Thirty-seven months post keratoplasty, the best-corrected visual acuity was 6/15 with clear graft and stable ocular surface. Herein, a case of limbal stem cell deficiency successfully managed by monolayer of cultured limbal epithelium is presented.

Item Type:Article
Source:Copyright of this article belongs to Optometrists Association Australia.
Keywords:Limbal Stem Cell Culture; Ocular Surface Disorder; Stratification
ID Code:25011
Deposited On:01 Dec 2010 12:18
Last Modified:08 Jun 2011 06:11

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