Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA

Saran, Shweta ; Nakao, Hajime ; Tasaka, Masao ; Iida, Hidetoshi ; Tsuji, Frederick I. ; Nanjundiah, Vidyanand ; Takeuchi, Ikuo (1994) Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA FEBS Letters, 337 (1). pp. 43-47. ISSN 0014-5793

[img]
Preview
PDF - Publisher Version
565kB

Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...

Related URL: http://dx.doi.org/10.1016/0014-5793(94)80626-8

Abstract

A new method is described for measuring intracellular free calcium concentrations, [(Ca2+i)], in the cells of Dictyostelium discoideum transformed with apoaequorin cDNA of the jellyfish, Aequorea victoria. Aequorin, a calcium-specific indicator, was regenerated in vivo from apoaequorin produced in the cells by incubation with coelenterazine. The results showed that [(Ca2+)i] in developing cells markedly increases at the aggregation stage and again at the culmination stage after a temporary drop at the migration stage. Except for the vegetative stage, the cells at all stages of development exhibit a sharp transient increase in [(Ca2+)i] upon stimulation with a cAMP (50 nM) pulse, high responses being observed at the migration and culmination stages. Separated prestalk cells of migrating slugs contain more than twice as much [(Ca2+)i] and show three times as large a response to cAMP stimulation as prespore cells.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:Dictyostelium Discoideum; Aequorin; Cyclic AMP; Calcium; Cell Differentiation
ID Code:24907
Deposited On:01 Dec 2010 12:29
Last Modified:17 May 2016 08:29

Repository Staff Only: item control page