Cloning of chicken globin cDNA in bacterial plasmids

Padayatty, Joseph ; Cummings, Ian ; Manske, Charles L. ; Higuchi, Russel ; Woo, Savio ; Salser, Winston (1981) Cloning of chicken globin cDNA in bacterial plasmids Gene, 13 (4). pp. 417-422. ISSN 0378-1119

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Chicken globin cDNA was prepared from mRNA isolated from phenylhydrazine-treated chicken reticulocytes, and cloned into the tetracycline-resistance gene of plasmid pMB9. Restriction enzyme analysis indicates that there are at least two classes of clones isolated. Nucleotide sequence data have shown that these classes corresponded to the chicken α- and β-globin genes.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Recombinant DNA; Restriction Endonuclease Mapping; DNA Sequencing; Hemoglobin mRNAs
ID Code:24720
Deposited On:30 Nov 2010 09:22
Last Modified:11 Jun 2011 11:04

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