Estimation of mineral-adhered biomass of Thiobacillus ferrooxidans by protein assay-some problems and remedies

Karan, Goutam ; Natarajan, K. A. ; Modak, J. M. (1996) Estimation of mineral-adhered biomass of Thiobacillus ferrooxidans by protein assay-some problems and remedies Hydrometallurgy, 42 (2). pp. 169-175. ISSN 0304-386X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/030438...

Related URL: http://dx.doi.org/10.1016/0304-386X(95)00080-Z

Abstract

Enumeration of adhered cells of Thiobacillus ferrooxidans on sulphide minerals through protein assay poses problems due to interference from dissolved mineral constituents. The manner in which sulphide minerals such as pyrite, chalcopyrite, sphalerite, arsenopyrite and pyrrhotite interfere with bacterial protein estimation is demonstrated. Such interferences can be minimised either through dilution or addition of H2O2 to the filtrate after hot alkaline digestion of the biotreated mineral samples.

Item Type:Article
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ID Code:24488
Deposited On:29 Nov 2010 08:48
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