High-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in Basmati rice (Oryza sativa)

Archak, Sunil ; Lakshminarayanareddy, V. ; Nagaraju, Javaregowda (2007) High-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in Basmati rice (Oryza sativa) Electrophoresis, 28 (14). pp. 2396-2405. ISSN 0173-0835

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Official URL: http://www3.interscience.wiley.com/journal/1142804...

Related URL: http://dx.doi.org/10.1002/elps.200600646

Abstract

Basmati rice is a very special type of aromatic rice known world-wide for its extra long grains and pleasant and distinct aroma. Traditional Basmati rice cultivars, confined to Indo-Gangetic regions of the Indian subcontinent, are often reported to be adulterated with crossbred Basmati varieties and long-grain non-Basmati varieties in the export market. At present, there is no commercial scale technology to reliably detect adulteration. We report here a CE-based multiplex microsatellite marker assay for detection as well as quantification of adulteration in Basmati rice samples. The single-tube assay multiplexes eight microsatellite loci to generate variety-specific allele profiles that can detect adulteration from 1% upwards. The protocol also incorporates a quantitative-competitive PCR-based analysis for quantification of adulteration. Accuracy of quantification has been shown to be ±1.5%. The experiments used to develop and validate the methodology are described.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:Adulteration; Basmati Rice; CE; Microsatellite Markers; Multiplex Assay; PCR
ID Code:24332
Deposited On:29 Nov 2010 09:10
Last Modified:17 May 2016 08:03

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