DNA fingerprinting in silkworm Bombyx mori using banded krait minor satellite DNA-derived probe

Abstract

The genomic DNA from thirteen different ecotypes and inbred lines of silkworm, Bombyx mori, were analyzed by digesting with BstNI and HinfI restriction enzymes followed by hybridization with banded krait minor satellite DNA (Bkm)-2(8) minisatellite probe. The DNA fingerprinting revealed 9-31 discrete intense bands, some of which were ecotype/inbred line-specific. Individual specific DNA fingerprints in two representative genotypes and their F₁ hybrid offspring were also obtained. Individuals of a given parental line showed very similar profiles and the hybrid offspring showed the combined profile of both parents. The presence of bands specific to diapausing and non-diapausing strains and to particular genotypes indicate their potential use for marker-assisted breeding and varietal identification.