Existence of single-strand interruptions in the genomic DNA of mycobacteriophage I3

Bramham Reddy, A. ; Gopinathan, K. P. (1986) Existence of single-strand interruptions in the genomic DNA of mycobacteriophage I3 FEMS Microbiology Letters, 37 (2). pp. 163-167. ISSN 0378-1097

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/037810...

Abstract

The genomic DNA of mycobacteriophage I3 harbours single-strand interruptions and is susceptible to degradation by nuclease S1, leading to random fragmentation. The native DNA serves as an efficient template for DNA polymerase I showing the presence of free 3'-OH termini which provide the priming points within the genome. The single-strand regions show no specificity for the 5'-end nucleotides flanking these interruptions and all 4 nucleotides are present with a probability equivalent to their percentage in the total DNA. The single-strand interruptions are an intrinsic character of this genome and are present irrespective of the host on which the phage is propagated.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Microbiological Societies.
Keywords:Bacteriophage; Mycobacteriophage I3; DNA Polymerase I Genome; Single-strand Interruptions
ID Code:23669
Deposited On:26 Nov 2010 08:50
Last Modified:02 Jun 2011 06:16

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