Transfection of Mycobacterium smegmatis SN2 with mycobacteriophage I3

Sadashiva Karnik, S. ; Gopinathan, K. P. (1983) Transfection of Mycobacterium smegmatis SN2 with mycobacteriophage I3 Archives of Microbiology, 136 (4). pp. 275-280. ISSN 0302-8933

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Mycobacterium smegmatis SN2 does not exhibit natural competence for the uptake of phage I3 DNA. Competence can artificially be induced by treatment with glycine or CaCl2, and the combination of both is even more effective. The efficiency of transfection can be improved by inclusion of protamine sulphate and heterologous RNA in the system. From 32P DNA uptake studies the major barrier for the entry of DNA has been found to be the complex cell wall. The efficiency of transfection calculated on the basis of fraction of DNA which has entered the cell is comparable to that of other bacterial systems. The phage development takes a longer time (7 h for one cycle) after transfection, as compared to infection (4 h).

Item Type:Article
Source:Copyright of this article belongs to Springer Verlag.
Keywords:Transfection; Mycobacterium smegmatis; Mycobacteriophage I3; Calcium Sensitization; Glycine Sensitization; Protamine Sulfate; Exogenous RNA; DNA Uptake
ID Code:23659
Deposited On:26 Nov 2010 08:48
Last Modified:02 Jun 2011 06:19

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