Deoxyribonucleic acid methylation in mycobacteria

Srivastava, R. ; Gopinathan, K. P. ; Ramakrishnan, T. (1981) Deoxyribonucleic acid methylation in mycobacteria Journal of Bacteriology, 148 (2). pp. 716-719. ISSN 0021-9193

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Deoxyribonucleic acid modification in six strains of mycobacteria was investigated. The presence of 5-methylcytosine in the virulent strain Mycobacterium tuberculosis H37Rv and its absence in the avirulent strain M. tuberculosis H37Ra and other saprophytic, fast-growing mycobacteria appear to be the salient features. However, deoxyribonucleic acid from M. smegmatis SN2 lysogenized with the temperature phage I3 showed the presence of 5-methylcytosine. All of the strains had N6-methyladenine.

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