Role of TATATAA element in the regulation of tRNAGly1 gene expression in Bombyx mori is position dependent

Sharma, Sujata ; Gopinathan, Karumathil P. (1996) Role of TATATAA element in the regulation of tRNAGly1 gene expression in Bombyx mori is position dependent Journal of Molecular Biology, 262 (4). pp. 396-406. ISSN 0022-2836

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00222...

Related URL: http://dx.doi.org/10.1006/jmbi.1996.0523

Abstract

Transcription of tRNA genes by RNA polymerase III is controlled by the internal conserved sequences within the coding region and the immediate upstream flanking sequences. A highly transcribed copy of glycyl tRNA gene tRNAGly1-1 fromBombyx moriis down regulated by sequences located much farther upstream in the region -150 to -300 nucleotides (nt), with respect to the +1 nt of tRNA. The negative regulatory effect has been narrowed down to a sequence motif 'TATATAA', a perfect consensus recognised by the TATA binding protein, TBP. This sequence element, when brought closer to the transcription start point, on the other hand, exerts a positive effect by promoting transcription of the gene devoid of othercisregulatory elements. The identity of the nuclear protein interacting with this 'TATATAA' element to TBP has been established by antibody and mutagenesis studies. The 'TATATAA' element thus influences the transcription of tRNA genes positively or negatively in a position-dependent manner either by recruitment or sequestration of TBP from the transcription machinery.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Pol III Transcription; Silkworm; Tata-binding Protein (TBP); Transcriptional Regulation; tRNAGly1genes
ID Code:23631
Deposited On:26 Nov 2010 08:44
Last Modified:02 Jun 2011 06:05

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