Iodine metabolism in dispersed pharyngeal and head kidney teleostean thyroid cells obtained by continuous trypsinization

Bhattacharya, Samir ; Das, R. H. ; Datta, Asoke G. (1976) Iodine metabolism in dispersed pharyngeal and head kidney teleostean thyroid cells obtained by continuous trypsinization General and Comparative Endocrinology, 30 (1). pp. 128-130. ISSN 0016-6480

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001664...

Related URL: http://dx.doi.org/10.1016/0016-6480(76)90073-3

Abstract

Free thyroid follicular cell suspension was prepared from pharyngeal and head kidney thyroid tissue of a teleost by a continuous trypsinization procedure. Single cell suspensions of both the regions retained the capacity of radioiodine uptake and metabolized it into iodoamino acids and thyroxine. Formation of iodotyrosines and thyroxine in free follicular cells was considerably stimulated by the addition of bovine TSH in the incubation medium and this stimulation was abolished by actinomycin D.

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