Anaerobic formation of succinate from glucose and bicarbonate in resting cells of Leishmania donovani

Chatterjee, Tapati ; Datta, Asoke G. (1973) Anaerobic formation of succinate from glucose and bicarbonate in resting cells of Leishmania donovani Experimental Parasitology, 33 (1). pp. 138-146. ISSN 0014-4894

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001448...

Related URL: http://dx.doi.org/10.1016/0014-4894(73)90019-2

Abstract

The cell suspension of Leishmania donovani incorporates 14CO2 resulting in the formation of [14C]-succinic acid under anaerobic conditions. The results showed that the [14C]-succinate formation from [1-14C]-glucose is much greater than that from [6-14C]-glucose. [14C-pyruvate] takes part in the production of succinic acid under anaerobic conditions without decarboxylation. The anaerobic formation of succinate appears to involve the production of malate, which is then converted to succinate via the reduction of fumarate by the reversal of the tricarboxylic acid cycle. Evidence indicated that the active species in this carboxylation reaction was CO2 although HCO3 was active to some extent.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Leishmania Donovani; Carbohydrates; Metabolism; Carbon Isotopes; Glucose; Pyruvate; Malate; Spectrophotometry; Chromatography; Guanosine-diphosphate; Guanosine-triphosphate; Adenosine Triphosphate; Pyrophosphate; Inorganic Phosphate; Nicotinamide Adenine Diphosphate; Inosine Triphosphate; Sodium Bicarbonate; Carbon Dioxide; Enzymes; Malic Enzyme (EC 1.1.1.40); EC 1.1.1.40 (malic Enzyme); Pyruvate Carboxylase (EC 6.4.1.1 ); EC 6.4.1.1 (pyruvate Carboxylase); Phosphoenolpyruvate Carboxykinase (EC 4.1.1.32); EC 4.1.1.32 (phosphoenolpyruvate Carboxykinase); Phosphoenolpyruvate Carboxylase (EC 4.1.1. 31); EC 4.1.1.31 (phosphoenolpyruvate Carboxylase); Phosphoenolpyruvate Carboxytransphosphorylase (EC 4.1.1.38); EC 4.1.1.38 (phosphoenolpyruvate Carboxytransphosphorylase
ID Code:23588
Deposited On:26 Nov 2010 08:38
Last Modified:31 May 2011 04:31

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