Chemical approach to aspirin hypersensitivity

Ramanjaneyulu, R. ; Madyastha, K. M. ; Bhattacharyya, P. K. (1981) Chemical approach to aspirin hypersensitivity Biochemical and Biophysical Research Communications, 101 (1). pp. 258-264. ISSN 0006-291X

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Rabbits and guinea pigs were immunized with functionalized aspirin-protein conjugates prepared by coupling 5-N-Succinylamino aspirin to BSA and BGG using a water soluble carbodiimide (EDC). Two populations of antibodies, one specific to functionalized aspirin and the other exclusively specific to salicylic acid were detected. These antibodies were fractionated and separated on affinity polymers suitably prepared with 5-N-succinylamino salicylic acid and 5-N-succinylamino-2-ethoxy benzoic acid as the ligands. The isolated and purified antibodies were electrophoretically homogeneous. The physico chemical interactions between the antibodies and the respective haptens were studied by radio-immunoassay, equilibrium dialysis and fluorescence quenching techniques.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:23467
Deposited On:25 Nov 2010 09:32
Last Modified:10 Jun 2011 06:42

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