Selective inhibition of cyclooxygenase-2 by C-phycocyanin, a biliprotein from Spirulina platensis

Madhava Reddy, C. ; Bhat, Vadiraja B. ; Kiranmai, G. ; Narsa Reddy, M. ; Reddanna, P. ; Madyastha, K. M. (2000) Selective inhibition of cyclooxygenase-2 by C-phycocyanin, a biliprotein from Spirulina platensis Biochemical and Biophysical Research Communications, 277 (3). pp. 599-603. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1006/bbrc.2000.3725

Abstract

We report data from two related assay systems (isolated enzyme assays and whole blood assays) that C-phycocyanin a biliprotein from Spirulina platensis is a selective inhibitor of cyclooxygenase-2 (COX-2) with a very low IC50 COX-2/IC50 COX-1 ratio (0.04). The extent of inhibition depends on the period of preincubation of phycocyanin with COX-2, but without any effect on the period of preincubation with COX-1. The IC50 value obtained for the inhibition of COX-2 by phycocyanin is much lower (180 nM) as compared to those of celecoxib (255 nM) and rofecoxib (401 nM), the well-known selective COX-2 inhibitors. In the human whole blood assay, phycocyanin very efficiently inhibited COX-2 with an IC50 value of 80 nM. Reduced phycocyanin and phycocyanobilin, the chromophore of phycocyanin are poor inhibitors of COX-2 without COX-2 selectivity. This suggests that apoprotein in phycocyanin plays a key role in the selective inhibition of COX-2. The present study points out that the hepatoprotective, anti-inflammatory, and anti-arthritic properties of phycocyanin reported in the literature may be due, in part, to its selective COX-2 inhibitory property, although its ability to efficiently scavenge free radicals and effectively inhibit lipid peroxidation may also be involved.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Nonsteroidal Anti-inflammatory Drugs; Phycocyanin; Reduced Phycocyanin; Phycocyanobilin; Cyclooxygenase-1; Cyclooxygenase-2; Inhibition
ID Code:23456
Deposited On:25 Nov 2010 09:29
Last Modified:10 Jun 2011 06:21

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