Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from spirulina platensis: protection against oxidative damage to DNA

Bhat, Vadiraja B. ; Madyastha, K. M. (2001) Scavenging of peroxynitrite by phycocyanin and phycocyanobilin from spirulina platensis: protection against oxidative damage to DNA Biochemical and Biophysical Research Communications, 285 (2). pp. 262-266. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1006/bbrc.2001.5195

Abstract

Peroxynitrite (ONOO- is known to inactivate important cellular targets and also mediate oxidative damage in DNA. The present study has demonstrated that phycocyanin, a biliprotein from spirulina platensis and its chromophore, phycocyanobilin (PCB), efficiently scavenge ONOO-, a potent physiological inorganic toxin. Scavenging of ONOO- by phycocyanin and PCB was established by studying their interaction with ONOO- and quantified by using competition kinetics of pyrogallol red bleaching assay. The relative antioxidant ratio and IC50 value clearly indicate that phycocyanin is a more efficient ONOO- scavenger than PCB. The present study has also shown that PCB significantly inhibits the ONOO--mediated single-strand breaks in supercoiled plasmid DNA in a dose-dependent manner with an IC50 value of 2.9 ± 0.6 μM. These results suggest that phycocyanin, has the ability to inhibit the ONOO--mediated deleterious biological effects and hence has the potential to be used as a therapeutic agent.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Phycocyanin; Phycocyanobilin; Peroxynitrite Scavenging; Pyrogallol Red; DNA Damage; Antioxidant
ID Code:23428
Deposited On:25 Nov 2010 09:14
Last Modified:10 Jun 2011 06:18

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