Partial sequence of the N-terminal portion of the protein of cowpea chlorotic mottle virus

Tremaine, J. H. ; Agrawal, H. O. ; Chidlow, J. (1972) Partial sequence of the N-terminal portion of the protein of cowpea chlorotic mottle virus Virology, 48 (1). pp. 245-254. ISSN 0042-6822

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/004268...

Related URL: http://dx.doi.org/10.1016/0042-6822(72)90131-6

Abstract

The action of trypsin on cowpea chlorotic mottle virus (CCMV) at pH 7.4 caused the release of 25 amino acid residues from the N-terminal of the protein. Separation of peptides released during this digestion on cation exchange resins and determination of their N-termini gave 6 major peptides: the N-terminal peptide CH3---C(=0)--- NH---(Lys, Thr2, Ser, Gly2, Val2); Ala---Ala---Ala---Arg; Asn---Thr---Arg; Ala---Gln---Arg; Leu---Thr---Arg; and Lys---Asn---Lys---Arg. Some of these peptides were cleaved by nontryptic enzymes in the digestion. Peptides cleaved by trypsin from chymotrypsintreated virus were Lys, Arg, and Asn---Thr---Arg. This information and the isolation of peptides containing more than one arginine were assessed to propose the partial sequence of 25 residues in the N-terminal of CCMV protein.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:233
Deposited On:17 Sep 2010 11:00
Last Modified:10 May 2011 05:20

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