Pillai, Shiv ; Surolia, A. ; Bachhawat, B. K. (1979) Critical density for protein-protein monoconjugation on a polysaccharide matrix Biochimica et Biophysica Acta (BBA) - Protein Structure, 580 (1). pp. 216-219. ISSN 0005-2795
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000527...
Related URL: http://dx.doi.org/10.1016/0005-2795(79)90212-5
Abstract
The galactose binding toxin (RCA||) from Ricinuscommunis was affinity-immobilised at varying densities on a polysaccharide matrix and reacted with glutaraldehyde. The critical density below which inter-molecular cross-links were not formed was determined. At this density RCA|| was monoconjugated to lysozyme. This approach could serve as a prototype for enzyme-lectin and enzyme-antipolysaccharide antibody monoconjugation.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Protein-protein Monoconjugation; Polysaccharide Matrix; Lectin; Glutaraldehyde; (R. Communis) |
| ID Code: | 2284 |
| Deposited On: | 07 Oct 2010 11:37 |
| Last Modified: | 14 Jun 2011 06:18 |
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